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Summary | The content, titled "Remarks on Methods for Retroviral Isolation," discusses the limitations of current methods for isolating retroviruses, particularly in relation to HIV/AIDS research. The author highlights the lack of scientific evidence demonstrating the direct isolation of retroviruses from AIDS patients using electron microscopy or molecular markers. The content questions the validity of the HIV/AIDS hypothesis and criticizes the reliance on HIV "markers" in research and clinical trials. It also mentions the use of antiviral drugs and the measurement of "viral load" in treated patients. Overall, the content suggests that the current methods and markers used in HIV/AIDS research may be flawed and calls for a reevaluation of the hypothesis.
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By Etienne de Harven
Continuum Spring 1998
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Dr. Etienne de Harven is emeritus Professor of Pathology, University of Toronto. He worked in electron microscopy (EM) primarily on the ultrastructure of retroviruses throughout his professional career of 25 years at the Sloan Kettering Institute in New York and 13 years at the University of Toronto. In 1956 he was the first to report on the EM of the Friend virus in murine (mouse) leukemia, and in 1960, to coin the word "budding" to describe steps of virus assembly on cell surfaces. He will deliver a speech at the 12th World AIDS Conference in Geneva (June 28-July 3) at the session "HIV-testing: Open Questions about Specificity".
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Obviously, the HIV/AIDS hypothesis has to be scientifically reappraised.(15) And, most urgently, the funding for AIDS research should no longer be restricted to laboratories working on an hypothesis which has never been proven. *
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Papadopulos-Eleopulos E, Turner VF, Papadimitriou JM, Causer D, Hedland-Thomas 13, Page B, 1994. A critical analysis of the HIV-T4-AIDS hypothesis. Genetica 95:5-24 Lanka, Stefan, 1994. Fehldiagnose AIDS? Wechselwirkung, Aachen, December, 48-53. 3 de Harven, E, 1974. remarks on the ultrastructure of type A, B and C virus particles. Advances in Virus Research 19: 221-264. Academic Press, Inc., publ., New York. Lwoff A, Horne R, Tournier P, 1962. Cold Spring Harbour Symposium on Quantitative Biology 27:51. 5 de Harven E, and Friend C, 1960. Further electron microscope studies of a mouse leukemia induced by cell-free filtrates. J. Biophysic. and Biochem. Cytol., vol 7, 747-752. Rockefeller University Press, New York de Harven E, 1965. Remarks on viruses, leukemia and electron microscopy. In Methodological approaches to the study of leukemias. V Defendi, edit., The Wistar Institute Press publ, Philadelphia, pp147-156 de Harven E, 1965. Viremia in Friend leukemia: the electron microscope approach to the problem Pathologie-Biologie,13:125-134 de Harven E, 1998. Pioneer deplores "HIV". Continuum vol 5, page 24 Sinoussi F, Mendiola L, Chermann JC, 1973. Purification and partial differentiation of the particles of murine sarcoma virus (M.MSV) according to their sedimentation rates in sucrose density gradients. Spectra 4:237-24 Papadopulos-Eleopulos E, Turner VF and Papadimitnou JM, 1993. Is a positive Western Blot proof of HIV infection? Bio/Technology 11:696-707 Todak C, Klein E, Lange M et al., 1991. A clinical appraisal of the p24 antigen test. International Conference on AIDS, vol 1, Florence, Italy Varmus H, 1987. Reverse transcription Sci. Am. 257:48-54 Gluschankof P. Mondor I, Gelderblom HR, and Sattentau QJ, 1997. Cell Membrane vesicles are a major contaminant of gradient-ennched human immunodeficiency virus type-l preparations. Virology 230:125-133 Bess JW Jr., Gorelick WJ, Bosche WJ, Henderson LE, and Arthur LO, 1997. Microvesicles are a source of contaminating cellular proteins found in purified HIV-I preparations. Virology 230:134-144 Farber, C, 1992. Fatal distraction, Spin Magazine, June 1992 Philpott P, 1997. The isolation question. Reappraising AIDS, vol 5 number 6, 1-12
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Author's address:
Dr Etienne de Harven
le Mas Pitou,
2879 Route de Grasse
06530 Saint Cezaire sur Siagne, France